In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood

Intravia, Jessica and Allen, Donald A. and Durant, Thomas JS. and McCarthy, Mary Beth R. and Russell, Ryan and Beitzel, Knut and Cote, Mark P. and Dias, Feliciano and Mazzocca, Augustus D. (2014) In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood. M.L.T.J. Muscles, Ligaments and Tendons Journal, 4 (1). pp. 79-84. ISSN 2240-4554

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Abstract

Purpose. This study investigates the antibacterial properties of two different platelet-rich plasma (PRP) platelet concentration preparations (PRPLP and PRPHP) through a time-kill assay. Methods. Two different PRP preparations - a single spin process yielding lower white blood cells and platelet concentration (PRPLP) and one yielding high platelet and white blood cell concentration (PRPHP) - were obtained from 2 individuals. PRPLP, PRPHP, phosphate buffered saline (PBS), whole blood and Cefazolin were added to experimental reaction tubes, each containing a single bacterial inoculum of Staphylococcus aureus (S.aureus), Staphylococcus epidermidis (S. epi), methicillin-resistant Staphylococcus aureus (MRSA) or Propionibacterium acnes (P. acnes). Two dilutions (1:1,000, and 1:10,000) were plated in duplicate tubes, along with positive (blood and PBS) and negative (Cefazolin) controls and assessed at five time points (0, 1, 4, 8 and 24 hours). Results. After centrifugation, platelet count of PRPLP was 386 ± 65.5 x 103/?L and PRPHP was 867 ± 234.4 x 103/?L. Both PRP products showed a significant decrease (p<0.05) in bacterial growth at 8 hours compared to whole blood. Conclusion. The application of PRPLP and PRPHP showed a significant decrease in bacterial growth after 8 hours for S. aureus, S. epi, MRSA and P. acnes compared to the whole blood control group. S.epi, MRSA, and P. acnes also showed a significant decrease in bacterial growth after 24 hours. Despite differences in platelet concentration and WBC concentration, no difference in antibacterial activity was seen between the two preparations

Item Type:	Article
Uncontrolled Keywords:	Antibacterial, PRP, platelet-rich plasma, antimicrobial
Subjects:	600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.7 Malattie del sistema muscoloscheletrico
Depositing User:	Danilo Dezzi
Date Deposited:	11 Nov 2014 11:27
Last Modified:	11 Nov 2014 11:27
URI:	http://eprints.bice.rm.cnr.it/id/eprint/10003

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