Further considerations on in vitro skeletal muscle cell death

Battistelli, Michela and Salucci, Sara and Burattini, Sabrina and Elisabetta Falcieri, Elisabetta (2013) Further considerations on in vitro skeletal muscle cell death. M.L.T.J. Muscles, Ligaments and Tendons Journal, 3 (4). pp. 267-274. ISSN 2240-4554

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Abstract

The present review discusses the apoptotic behavior induced by chemical and physical triggers in C2C12 skeletal muscle cells, comparing myoblast to myotube sensitivity, and investigating it by means of morphological, biochemical and cytofluorimetric analyses. After all treatments, myotubes, differently from myoblasts, showed a poor sensitivity to cell death. Intriguingly, in cells exposed to staurosporine, etoposide and UVB radiation, apoptotic and normal nuclei within the same fibercould be revealed. The presence of nuclear-dependent “territorial” death domains in the syncytium could explain a delayed cell death of myotubes compared to mononucleated cells. Moreover, autophagic granules abundantly appeared in myotubes after each treatment. Autophagy could protect muscle cell integrity against chemical and physical stimuli, making C2C12 myotubes, more resistant to cell death induction

Item Type:	Article
Uncontrolled Keywords:	Autophagy, cell death, chemical triggers, myoblasts, myotubes, physical trigger
Subjects:	600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.7 Malattie del sistema muscoloscheletrico
Depositing User:	Danilo Dezzi
Date Deposited:	14 Nov 2014 16:50
Last Modified:	14 Nov 2014 16:50
URI:	http://eprints.bice.rm.cnr.it/id/eprint/10091

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