SEM evaluation of human gingival fibroblasts growth onto CAD/CAM zirconia and veneering ceramic for zirconia

Zizzari, Vincenzo and Borelli, Bruna and De Colli, Marianna and Tumedei, Margherita and Di Iorio, Donato and Zara, Susi and Sorrentino, Roberto and Cataldi, Amelia and Gherlone, Enrico Felice and Zarone, Fernando and Tetè, Stefano (2013) SEM evaluation of human gingival fibroblasts growth onto CAD/CAM zirconia and veneering ceramic for zirconia. Annali di stomatologia, 4 (3/4). pp. 244-249. ISSN 1971-1441

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To evaluate the growth of Human Gingival Fibroblasts (HGFs) cultured onto sample discs of CAD/CAM zirconia and veneering ceramic for zirconia by means of Scanning Electron Microscope(SEM) analysis at different experimental times. Methods: A total of 26 experimental discs, divided into 2 groups, were used: Group A) CAD/CAM zirconia(3Y-TZP) discs (n=13); Group B) veneering ceramic for zirconia discs (n=13). HGFs were obtained from human gingival biopsies, isolated and placed in culture plates. Subsequently, cells were seeded on experimental discs at 7,5x103/cm2 concentration and cultured for a total of 7 days. Discs were processed for SEM observation at 3h, 24h, 72h and 7 days. Results: In Group A, after 3h, HGFs were adherent to the surface and showed a flattened profile. The disc surface covered by HGFs resulted to be wider in Group A than in Group B samples. At SEM observation, after 24h and 72h, differences in cell attachment were slightly noticeable between the groups, with an evident flattening of HGFs on both surfaces. All differences between Group A and group B became less significant after 7 days of culture in vitro. Conclusions: SEM analysis of HGFs showed differences in terms of cell adhesion and proliferation, especially in the early hours of culture. Results showed a better adhesion and cell growth in Group A than in Group B, especially up to 72h in vitro. Differences decreased after 7 days, probably because of the rougher surface of CAD/CAM zirconia, promoting better cell adhesion, compared to the smoother surface of veneering ceramic.

Item Type: Article
Uncontrolled Keywords: CAD/CAM zirconia, veneering ceramic, human gingival fibroblasts, scanning electron microscope, zirconia cell attachment, ceramic cell attachment
Subjects: 600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 610.7 Medicina - Educazione e Insegnamento; Medicina - Ricerca
Depositing User: Marina Spanti
Date Deposited: 08 Jul 2015 14:49
Last Modified: 08 Jul 2015 15:25

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