Shearer, Tom and Rawson, Shelley and Castro, Simon Joseph and Balint, Richard and Bradley, Robert Stephen and Lowe, Tristan and Vila-Comamala, Joan and Lee, Peter David and Cartmell, Sarah Harriet (2014) X-ray computed tomography of the anterior cruciate ligament and patellar tendon. M.L.T.J. Muscles, Ligaments and Tendons Journal, 4 (2). pp. 238-244. ISSN 2240-4554
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Abstract
The effect of phosphotungstic acid (PTA) and iodine solution (IKI) staining was investigated as a method of enhancing contrast in the X-ray computed tomography of porcine anterior cruciate ligaments (ACL) and patellar tendons (PT). We show that PTA enhanced surface contrast, but was ineffective at penetrating samples, whereas IKI penetrated more effectively and enhanced contrast after 70 hours of staining. Contrast enhancement was compared when using laboratory and synchrotron based X-ray sources. Using the laboratory source, PT fascicles were tracked and their alignment was measured. Individual ACL fascicles could not be identified, but identifiable features were evident that were tracked. Higher resolution scans of fascicle bundles from the PT and ACL were obtained using synchrotron imaging techniques. These scans exhibited greater contrast between the fascicles and matrix in the PT sample, facilitating the identification of the fascicle edges; however, it was still not possible to detect individual fascicles in the ACL.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | anterior cruciate ligament, contrast enhancement, fascicle, patellar tendon, staining, X-ray tomography |
| Subjects: | 600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 617 Rami vari della medicina; Chirurgia > 617.1 Ferite e lesioni |
| Depositing User: | Chiara D'Arpa |
| Date Deposited: | 20 May 2015 14:20 |
| Last Modified: | 20 May 2015 14:20 |
| URI: | http://eprints.bice.rm.cnr.it/id/eprint/10925 |
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