Angiogenic and innate immune responses triggered by PRP in tendon cells are not modified by hyperuricemia

Andia, Isabel and Rubio-Azpeitia, Eva (2014) Angiogenic and innate immune responses triggered by PRP in tendon cells are not modified by hyperuricemia. M.L.T.J. Muscles, Ligaments and Tendons Journal, 4 (3). pp. 292-297. ISSN 2240-4554

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Background: hyperuricemia is becoming a critical medical problem, and a current focus of research. Uric acid is also a death cell associated stressor that may trigger innate immune responses via the synthesis of inflammatory and angiogenic proteins. Purpose: to investigate the angiogenic/inflammatory protein profile of tendon cells treated with Platelet Rich Plasma (PRP), and to assess if there are any differences in synthesis of angiogenic/inflammatory cytokines between PRP-treated or hyperuricemic PRP-treated cells. Methods: tendon cells were treated with PRP or hyperuricemic PRP and cell culture supernatants examined using glass based protein arrays for inflammation and angiogenesis. Relevant proteins were subsequently quantified by ELISA or EASIA methods. Results: the impact of PRP on angiogenesis and inflammation is evidenced by relevant cytokine synthesis including: Monocyte Chemoattractant Protein (MCP-1/CCL2), Regulated upon Activation Normally T cells Expressed and Secreted (RANTES/CCL5), IL-6/CXCL6, IL-8/CXCL8, Vascular Endothelial Growth Factor (VEGF), Growth Regulated Protein (GRO-a/CXCL1) and Hepatocyte Growth Factor (HGF). IL-1beta was not detected in these conditions. Taken together these data suggest an initial angiogenetic and innate immune responses driven by chemokines that is not altered by the presence of hyperuricemia, at this point, except for IL-8 secretion, p= 0.042.

Item Type: Article
Uncontrolled Keywords: PRP, uric acid, tenocyte, chemokines, growth factors
Subjects: 600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 617 Rami vari della medicina; Chirurgia > 617.1 Ferite e lesioni
Depositing User: Chiara D'Arpa
Date Deposited: 03 Jun 2015 10:02
Last Modified: 03 Jun 2015 10:02

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