Paternal alcohol exposure in mice alters brain NGF and BDNF and increases ethanol-elicited preference in male offspring

Ceccanti, Mauro and Coccurello, Roberto and Carito, Valentina and Ciafrè, Stefania and Ferraguti, Giampiero and Giacovazzo, Giacomo and Mancinelli, Rosanna and Tirassa, Paola and Chaldakov, George N. and Pascale, Esterina and Ceccanti, Marco and Codazzo, Claudia and Fiore, Marco (2015) Paternal alcohol exposure in mice alters brain NGF and BDNF and increases ethanol-elicited preference in male offspring. Addiction Biology, 21 (4). pp. 776-787. ISSN 1355-6215

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Ethanol (EtOH) exposure during pregnancy induces cognitive and physiological deficits in the offspring. However, the role of paternal alcohol exposure (PAE) on offspring EtOH sensitivity and neurotrophins has not received much attention. The present study examined whether PAE may disrupt nerve growth factor (NGF) and/or brain-derived neurotrophic factor (BDNF) and affect EtOH preference/rewarding properties in the male offspring. CD1 sire mice were chronically addicted for EtOH or administered with sucrose. Their male offsprings when adult were assessed for EtOH preference by a conditioned place preference paradigm. NGF and BDNF, their receptors (p75NTR, TrkA and TrkB), dopamine active transporter (DAT), dopamine receptors D1 and D2, pro-NGF and pro-BDNF were also evaluated in brain areas. PAE affected NGF levels in frontal cortex, striatum, olfactory lobes, hippocampus and hypothalamus. BDNF alterations in frontal cortex, striatum and olfactory lobes were found. PAE induced a higher susceptibility to the EtOH rewarding effects mostly evident at the lower concentration (0.5 g/kg) that was ineffective in non-PAE offsprings. Moreover, higher ethanol concentrations (1.5 g/kg) produced an aversive response in PAE animals and a significant preference in non-PAE offspring. PAE affected also TrkA in the hippocampus and p75NTR in the frontal cortex. DAT was affected in the olfactory lobes in PAE animals treated with 0.5 g/kg of ethanol while no differences were found on D1/D2 receptors and for pro-NGF or pro-BDNF. In conclusion, this study shows that: PAE affects NGF and BDNF expression in the mouse brain; PAE may induce ethanol intake preference in the male offspring.

Item Type: Article
Uncontrolled Keywords: Ethanol intake preference, neurotrophin, paternal alcohol exposure
Subjects: 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia
Depositing User: PhD Roberto Coccurello
Date Deposited: 21 Dec 2016 14:05
Last Modified: 21 Dec 2016 14:05

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