Picollo, F. (2013) Amperometric detection of quantal catecholamine secretion from individual cells by an ion beam microfabricated single crystalline diamond biosensor. Il nuovo cimento C, 36 (4). pp. 153-160. ISSN 1826-9885
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Abstract
It is shown that buried graphitic channels fabricated in monocrystalline diamond by selective damage induced by focused MeV ions, can be considered an effective alternative to the commonly used carbon-fibers to detect the catecholamine release from cells as individual secretory granules discharge their contents during the process of quantal exocytosis. Quantal secretory responses have been measured from stimulated chromaffin cells, which were positioned on the graphitic microelectrode, polarized to +800 mV. Sequences of amperometric spikes started after cell stimulation with the KCl solution, with amplitudes well above the background noise within the range of 8–180 pA and comparable with signals obtained by conventional carbon fiber electrodes.
| Item Type: | Article |
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| Uncontrolled Keywords: | Lithography, masks and pattern transfer ; Diamond/nanocarbon composites ; Applied neuroscience |
| Subjects: | 500 Scienze naturali e Matematica > 530 Fisica |
| Depositing User: | Marina Spanti |
| Date Deposited: | 06 May 2020 15:32 |
| Last Modified: | 06 May 2020 15:32 |
| URI: | http://eprints.bice.rm.cnr.it/id/eprint/18295 |
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