Degradation of Aflatoxin B1 by a Sustainable Enzymatic Extract from Spent Mushroom Substrate of Pleurotus eryngii

Branà, Maria Teresa and Sergio, Lucrezia and Haidukowski, Miriam and Logrieco, Antonio F. and Altomare, Claudio (2020) Degradation of Aflatoxin B1 by a Sustainable Enzymatic Extract from Spent Mushroom Substrate of Pleurotus eryngii. Toxins, 12 (1). pp. 1-13. ISSN 2072-6651

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Ligninolytic enzymes from white-rot fungi, such as laccase (Lac) and Mn-peroxidase (MnP), are able to degrade aflatoxin B1 (AFB1), the most harmful among the known mycotoxins. The high cost of purification of these enzymes has limited their implementation into practical technologies. Every year, tons of spent mushroom substrate (SMS) are produced as a by-product of edible mushroom cultivation, such as Pleurotus spp., and disposed at a cost for farmers. SMS may still bea source of ligninolytic enzymes useful for AFB1 degradation. The in vitro AFB1-degradative activity of an SMS crude extract (SMSE) was investigated. Results show that: (1) in SMSE, high Lac activity (4 U g−1 dry matter) and low MnP activity (0.4 U g−1 dry matter) were present; (2) after 1 d of incubation at 25 °C, the SMSE was able to degrade more than 50% of AFB1, whereas after 3 and 7 d of incubation, the percentage of degradation reached the values of 75% and 90%, respectively; (3) with increasing pH values, the degradation percentage increased, reaching 90% after 3 d at pH 8. Based on these results, SMS proved to be a suitable source of AFB1 degrading enzymes and the use of SMSE to detoxify AFB1 contaminated commodities appears conceivable.

Item Type: Article
Additional Information: Acknowledgments The authors are thankful to the De Biase s.r.l. mushroom production company (Castellaneta, Italy) for providing SMS, to Roberto Schena for his technical assistance, and to Pasquale Del Vecchio for the assistance on journals publication policy and copyright.
Uncontrolled Keywords: Pleurotus eryngi; king oyster mushroom; spent mushroom substrate; crude extract; aflatoxins; laccase; feed detoxification
Subjects: 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia > 579 Microrganismi, funghi, alghe. Classificare qui la Microbiologia, i Protista, le protofite, le Thallobionta (Thallophyta); la Storia naturale e la Biologia descrittiva di microrganismi, funghi, alghe; le opere interdisciplinari su microrganismi, funghi
Depositing User: Mr Pasquale Del Vecchio
Date Deposited: 28 Jan 2020 17:08
Last Modified: 28 Jan 2020 17:13

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