Bruni, L. (2019) A view of DNA short sequences rich in guanines like potassium biosensor. Il nuovo cimento C, 42 (5). pp. 1-11. ISSN 1826-9885
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Abstract
Pu23 and Pu27, single strand DNA sequences rich in guanines, can fold forming a G-quadruplex, in the presence of Na+ and K+. A G-quadruplex is a DNA secondary structure made up of at least two tetrads, an array made of four guanines, stacked on each other. Pu27, the 27-base–long wild-type sequence, is the c-myc transcription repressor. Pu23 is a 23-base–long mutant. In this work Pu27 and Pu23 are tested as watery K+ biosensors, thanks to their K+ affinity, even when Na+ exceeds the K+ concentration. The Pu27 and Pu23 biosensors are assayed in DMEM treated with a potassium solution. DMEM is a common medium where cancer cells can grow. This work shows that Pu27 and Pu23 can sense K+ into treated DMEM folding, according to the G4 parallel conformation. The titration with Na+ does not affect the Pu27 structural stability while, on the contrary, Pu23 displays a less stable folding. At fixed Na+ concentration, Pu27 shows higher sensitivity and better resolution for K+ detection with respect to Pu23; nevertheless, both sequences seem not to be adequate to measure a K+ concentration difference of about 1 mM.
| Item Type: | Article |
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| Subjects: | 500 Scienze naturali e Matematica > 530 Fisica |
| Depositing User: | Marina Spanti |
| Date Deposited: | 29 Jan 2021 11:28 |
| Last Modified: | 29 Jan 2021 11:28 |
| URI: | http://eprints.bice.rm.cnr.it/id/eprint/20677 |
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