Investigating nanoscale chromatin alterations involved in neuroblastoma transformation by optical nanoscopy

Baldini, F. and Cainero, I. and Cuneo, L. and Oneto, M. and Gatta, E. (2022) Investigating nanoscale chromatin alterations involved in neuroblastoma transformation by optical nanoscopy. Il nuovo cimento C, 45 (6). pp. 1-4. ISSN 1826-9885

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Abstract

Neuroblastoma (NB) is the most common extracranial solid tumor in childhood and is characterized by remarkable heterogeneity. This work aims to characterize changes in chromatin nanoscale architecture being associated with NB transformation. We employed an NB cell line overexpressing the non-coding RNA NDM29, which promotes cell differentiation toward a neuronal phenotype. The nuclear shape, volume and architecture were assessed in both malignant and neuronlike cells by confocal microscopy. Moreover, heterochromatin and euchromatin organization was investigated by stimulated emission depletion (STED) microscopy. The results showed that the nuclei of neuron-like cells have a reduced volume and a more elongated shape compared to those of malignant cells and a different spatial arrangement of euchromatin and heterochromatin. Altogether these data point to an alteration of nuclear organization associated to NB, paving the way towards a better comprehension of the disease.

Item Type:	Article
Subjects:	500 Scienze naturali e Matematica > 530 Fisica
Depositing User:	Marina Spanti
Date Deposited:	20 Sep 2022 09:13
Last Modified:	20 Sep 2022 09:13
URI:	http://eprints.bice.rm.cnr.it/id/eprint/22080

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