Low-Cost Synthesis of Smart Biocompatible Graphene Oxide Reduced Species by Means of GFP

Masullo, Tiziana and Armata, Nerina and Pendolino, Flavio and Colombo, Paolo and Celso, Fabrizio Lo and Mazzola, Salvatore and Cuttitta, Angela (2016) Low-Cost Synthesis of Smart Biocompatible Graphene Oxide Reduced Species by Means of GFP. Applied Biochemistry and Biotechnology, 178 (3). pp. 462-473. ISSN 0273-2289

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Official URL: http://dx.doi.org/10.1007/s12010-015-1887-5


The aim of this work is focused on the engineering of biocompatible complex systems composed of an inorganic and bio part. Graphene oxide (GO) and/or graphite oxide (GtO) were taken into account as potential substrates to the linkage of the protein such as Anemonia sulcata recombinant green fluorescent protein (rAsGFP). The complex system is obtained through a reduction process between GO/GtO and rAsGFP archiving an environmentally friendly biosynthesis. Spectroscopic measurements support the formation of reduced species. In particular, photoluminescence shows a change in the activity of the protein when a bond is formed, highlighted by a loss of the maximum emission signal of rAsGFP and a redshift of the maximum absorption peak of the GO/GtO species. Moreover, the hemolysis assay reveals a lower value in the presence of less oxidized graphene species providing evidence for a biocompatible material.

Item Type: Article
Subjects: 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia > 572 Biochimica (Classificare qui la Citochimica, l'Istochimica, le opere d'insieme su Biochimica e Biofisica; le opere interdisciplinari sulle sostanze biochimiche, sulle macromolecole)
Depositing User: Dr Tiziana Masullo
Date Deposited: 13 Jul 2023 08:39
Last Modified: 13 Jul 2023 08:39
URI: http://eprints.bice.rm.cnr.it/id/eprint/22372

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