Structured illumination generation with an integrated optical chip

Calisesi, Gianmaria (2023) Structured illumination generation with an integrated optical chip. Il nuovo cimento C, 46 (3). pp. 1-8. ISSN 1826-9885

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Structured illumination is a widely used technique in fluorescence microscopy that allows for super-resolution imaging. In this paper, a brief introduction to the topic will be presented, together with a compact and integrated optical device that can generate and spatially translate a structured light pattern suitable for SIM microscopy. The device uses optical waveguides and directional couplers made with femtosecond laser micromachining. The beams are directed through an optical coupler to create the illumination pattern after interfering. The phase of the beams in the waveguides can be adjusted using thermal phase-shifters so that it is possible to shift the illumination pattern over the field of view of the microscope, enabling the acquisition of multiple phase images for SIM reconstruction without the need for additional optical elements. The effectiveness of the device is demonstrated by showing its use in a commercially available inverted microscope for superresolution imaging of Bovine Pulmonary Artery Endothelial Cells (BPAE Line) deposited on a commercial glass slide.

Item Type: Article
Subjects: 500 Scienze naturali e Matematica > 530 Fisica
Depositing User: Marina Spanti
Date Deposited: 29 Jan 2024 12:17
Last Modified: 29 Jan 2024 12:17

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