Single-tear proteomics for noninvasive biomarker discovery and precision medicine

Ponzini, E. and D’Ami, D. and Duse, A. and Santambrogio, C. (2023) Single-tear proteomics for noninvasive biomarker discovery and precision medicine. Il nuovo cimento C, 46 (5). pp. 1-4. ISSN 1826-9885

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In the last decades, the lacrimal fluid has gained increasing interest as a potential source of biomarkers, thanks to its accessibility, moderate complex- ity, and responsiveness to physiopathological conditions. Despite the still limited number of studies, tear investigation, in particular single-tear analysis, could of- fer unique contributions to the identification of noninvasive and easily accessible biomarkers, and to the development of a feasible approach to precision medicine. High-performance liquid chromatography-mass spectrometry (LC-MS) has led to promising approaches to tear proteomics, despite the intrinsic limitations in the sam- ple amounts that can be collected. By ultrahigh-resolution, shotgun proteomics, we developed an effective analytical pipeline for single-tear analysis and we analyzed the tear fluid of 23 healthy volunteers, achieving high-confidence identification of 890 proteins. This study demonstrates the feasibility of single-tear quantitative pro- teomics, highlighting the unique contributions that this unconventional body fluid can offer to personalized approaches in biomedicine.

Item Type: Article
Subjects: 500 Scienze naturali e Matematica > 530 Fisica
Depositing User: Marina Spanti
Date Deposited: 15 Mar 2024 15:45
Last Modified: 15 Mar 2024 15:45

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