No synergistic effect of mesenchymal stem cells and exercise on functional recovery following sciatic nerve transection

Wang, John and Yang, Chen-Chia and Chen, Shyh-Chang and Hsieh, Yueh-Ling (2010) No synergistic effect of mesenchymal stem cells and exercise on functional recovery following sciatic nerve transection. Functional Neurology; New Trends in Interventional Neurosciences, 25 (1). pp. 33-43. ISSN 1971-3274

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Abstract

he present study examined whether transplantation of mesenchymal stem cells (MSCs) in combination with exercise would have synergistic effects leading to functional recovery that is greater than exercise alone. Sprague-Dawley rats received a sciatic nerve transection and were divided into four groups: denervated (control), denervated + exercise (control+Ex), denervated + MSC transplantation (MSC), and denervated + MSC transplantation + exercise (MSC+Ex). A volume of 1 x 105 of MSCs was injected into the lesion site in the MSC-treated groups, and culture medium in the control animals. Twelve hours after surgery, a swimming exercise regime was begun: 30 minutes/day for seven days in the MSC+Ex and control+Ex groups. Functional assessments including sciatic function index (SFI), vertical locomotor activity (VA), ankle activity (AA), and electrophysiological studies were performed to monitor the functional recovery. Histological analysis was performed to assess nerve continuity and myelination. No significant differences in SFI, VA, AA and electrophysiological studies were found between the MSC+Ex and control+Ex groups. Also, a morphological study revealed prominent axonal degeneration in the injured nerves of all animals. The results revealed that any synergistic effect of MSC transplantation on functional recovery of swimming exercise-treated transected nerve that may have existed was negligible

Item Type:	Article
Uncontrolled Keywords:	exercise, functional recovery, mesenchymal stem cells, sciatic nerve transection, synergistic effects
Subjects:	600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.8 Malattie del sistema nervoso e disturbi mentali (Classificare qui la Neuropsichiatria, la Neurologia) 600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.8 Malattie del sistema nervoso e disturbi mentali (Classificare qui la Neuropsichiatria, la Neurologia) > 616.85 Malattie varie del sistema nervoso e disturbi mentali di diverso genere > 616.856 Malattie dei nervi cranici, spinali, periferici; malattie del sistema nervoso autonomo (Include le neuralgie, le nevriti, la sciatica, la sindrome del tunnel carpale; i disturbi della sensibilità cutanea, i disturbi dell’olfatto e del gusto)
Depositing User:	Danilo Dezzi
Date Deposited:	25 Nov 2011 16:14
Last Modified:	25 Nov 2011 16:14
URI:	http://eprints.bice.rm.cnr.it/id/eprint/3522

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