Muscle extracellular matrix degradation and contractibility following tendon rupture and disuse

Zhang, Qia and Joshi, Sunil K. and Manzano, Givenchy and Lovett, David H. and Kim, Hubert T. and Liu, Xuhui (2013) Muscle extracellular matrix degradation and contractibility following tendon rupture and disuse. M.L.T.J. Muscles, Ligaments and Tendons Journal, 3 (1). pp. 35-41. ISSN 2240-4554

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Muscle extracellular matrix (ECM) plays an important role in maintaining muscular integrity and force transduction. However, the role of ECM in skeletal muscle atrophy remains unknown. In this study, we employed two clinically relevant mouse models of Achillotenotomy and hindlimb suspension to simulate Achilles tendon rupture and hindlimb disuse. The gastrocnemius was harvested following two weeks of treatment. We hypothesized that degradation of muscle ECM basement membrane lead to dysfunction of muscle contractility. Our results demonstrated a significant reduction of gastrocnemius single twitch force, isometric tetanic force, and contraction velocity following tendon rupture (p<0.001), but not disuse. Additionally, up-regulation of matrix metalloproteinase-2 (MMP-2) was observed only after tendon rupture (p=0.00234). These findings suggest that ECM remodeling and basement membrane degradation due to MMP-2 may be responsible for declined muscle contractibility. Inhibiting ECM degradation enzymes may be a potential treatment strategy for skeletal muscle atrophy after tendon rupture

Item Type: Article
Uncontrolled Keywords: Achillotenotomy, basement membrane, extracellular matrix
Subjects: 600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.7 Malattie del sistema muscoloscheletrico
Depositing User: Danilo Dezzi
Date Deposited: 17 Apr 2014 11:06
Last Modified: 17 Apr 2014 11:06

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