Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality

Bausset, Olivier and Magalon, Jeremy and Giraudo, Laurent and Louis, Marie-Laure and Serratrice, Nicolas and Frere, Corrine and Magalon, Guy and Dignat-George, Françoise and Sabatier, Florence (2014) Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality. M.L.T.J. Muscles, Ligaments and Tendons Journal, 4 (1). pp. 18-23. ISSN 2240-4554

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Abstract

The platelet-rich plasma (PRP) is an autologous biotherapy commonly used for its healing properties. Once activated, platelets released a real “cocktail” of growth factor and cytokines implied in numerous regenerative processes. However the impact of medical practices associated to PRP therapeutic use on platelets functionality remains poorly known. Objectives: we evaluated the in vitro effects of two commonly used local anesthetics (Xylocaine* and Naropin*) on PRP functionality. We also investigated the quantity and quality of PRP that passed through the smallest gauge needle commercialized. Materials and methods: PRP from 9 healthy volunteers were prepared using our previously described home made purification protocol. Platelet aggregation capacity was evaluated by aggregometry assays and the growth factor release was determined by ELISA after platelet activation. We also evaluated the platelet activation status, reactivity and stability of platelets by flow cytometry using the P-selectin expression marker. Results: the association of local anaesthetics with PRP injections resulted in a significant decrease of platelets functionality, assessed by their capacity of aggregating. Local anaesthetics did not interfere with the growth factor release. The different needle sizes and calibres tested for PRP injections did not influence the platelet functionality. Conclusions: the use of local anaesthetics to prevent pain during PRP injections could compromise the therapeutic potential of PRP. These results suggest using carefully local anaesthetics or limiting their use as often is possible. To minimize injection pain, we recommend using 30 G needles. These data will lead to clinical recommendations for painless and controlled PRP injections

Item Type:	Article
Uncontrolled Keywords:	Platelet-rich plasma, PRP, Platelet concentrate local anaesthetics, growth factors, tendinopathy, sport medicine, regenerative medicine
Subjects:	600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.7 Malattie del sistema muscoloscheletrico
Depositing User:	Danilo Dezzi
Date Deposited:	10 Nov 2014 16:19
Last Modified:	10 Nov 2014 16:19
URI:	http://eprints.bice.rm.cnr.it/id/eprint/9958

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