Filiciotto, Francesco and Giulia, Maricchiolo and Valentina, Corrias and Monica, Celi and Vincenzo, Maccarrone and Giuseppa, Buscaino and Lucrezia, Genovese Lab experimental setting for analysis of Mytilus galloprovincialis’ behaviour after wave-generated sounds exposure. Technical Report. IAMC-CNR, Capo Granitola. (Unpublished)
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Lab experimental setting for analysis of Mytilus galloprovincialis’ behaviour after wave-generated sounds exposure.pdf - Draft Version Download (6MB) | Preview |
Abstract
The present paper proposes an experimental lab setting and an innovative method in order to evaluate the behavioural reactions of Mediterranean mussels (Mytilus galloprovincialis) exposed to different acoustic stimuli. Ninety mussels were exposed for 30 minutes to one of five acoustic tones repeated in continuous or to the control condition. The tones lasted 1 s and differed in frequency band: Low frequencies-sweep (L, 0.1 - 5 kHz); Mid-Low frequencies-sweep (ML, 5 - 10 kHz); Mid frequencies-sweep (M, 10 - 20 kHz); Mid-High frequencies-sweep (MH, 20 - 40 kHz); High frequencies-sweep (H, 40 - 60 kHz). Experimental test sweeps were produced using a signal generator connected to underwater loudspeakers and a calibrated hydrophone was used to obtain acoustic recordings of sweeps and experimental tank background noise. An underwater camera, placed overhead the animals, recorded the mussels’ behaviours. A continuous visual sampling procedure, performed by an observatory, was adopted to detect the mussel gape rate and the outpouching of the muscular foot while an automatic analysis was performed using EthoVision XT 9.0 software. The behavioural parameters assessed automatically included changes in mobility status, movement, velocity and turn angle that are usually estimated in vertebrates and higher invertebrates.
| Item Type: | Monograph (Technical Report) |
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| Subjects: | 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia > 577 Ecologia |
| Depositing User: | Dr. Francesco Filiciotto |
| Date Deposited: | 09 Sep 2015 08:22 |
| Last Modified: | 09 Sep 2015 08:22 |
| URI: | http://eprints.bice.rm.cnr.it/id/eprint/11593 |
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