Lab experimental setting for analysis of Mytilus galloprovincialis’ behaviour after wave-generated sounds exposure

Filiciotto, Francesco and Giulia, Maricchiolo and Valentina, Corrias and Monica, Celi and Vincenzo, Maccarrone and Giuseppa, Buscaino and Lucrezia, Genovese Lab experimental setting for analysis of Mytilus galloprovincialis’ behaviour after wave-generated sounds exposure. Technical Report. IAMC-CNR, Capo Granitola. (Unpublished)

[img]
Preview
Text
Lab experimental setting for analysis of Mytilus galloprovincialis’ behaviour after wave-generated sounds exposure.pdf - Draft Version

Download (6MB) | Preview

Abstract

The present paper proposes an experimental lab setting and an innovative method in order to evaluate the behavioural reactions of Mediterranean mussels (Mytilus galloprovincialis) exposed to different acoustic stimuli. Ninety mussels were exposed for 30 minutes to one of five acoustic tones repeated in continuous or to the control condition. The tones lasted 1 s and differed in frequency band: Low frequencies-sweep (L, 0.1 - 5 kHz); Mid-Low frequencies-sweep (ML, 5 - 10 kHz); Mid frequencies-sweep (M, 10 - 20 kHz); Mid-High frequencies-sweep (MH, 20 - 40 kHz); High frequencies-sweep (H, 40 - 60 kHz). Experimental test sweeps were produced using a signal generator connected to underwater loudspeakers and a calibrated hydrophone was used to obtain acoustic recordings of sweeps and experimental tank background noise. An underwater camera, placed overhead the animals, recorded the mussels’ behaviours. A continuous visual sampling procedure, performed by an observatory, was adopted to detect the mussel gape rate and the outpouching of the muscular foot while an automatic analysis was performed using EthoVision XT 9.0 software. The behavioural parameters assessed automatically included changes in mobility status, movement, velocity and turn angle that are usually estimated in vertebrates and higher invertebrates.

Item Type: Monograph (Technical Report)
Additional Information: Au, W. W. L., Popper, A. N., Fay, R. J. (2000). Hearing by whales and dolphins, pp. 512. New York: Springer-Verlag. Budelmann, B. U. (1992a). Hearing in crustacea. In The evolutionary biology of hearing. (ed. D. B. Webster, R. R. Fay and A. N. Popper), pp. 131-140. New York: Springer-Verlag. Budelmann, B. U. (1992b). Hearing in non-arthropod invertebrates. In The evolutionary biology of hearing. (ed. D. B. Webster, R. R. Fay and A. N. Popper), pp. 141-155. New York: Springer- Verlag. Celi, M., Filiciotto, F., Parrinello, D., Buscaino, G., Damiano, A., Cuttitta, A., D’Angelo, S., Mazzola, S. and Vazzana, M. (2013). Physiological and agonistic behavioural response of Procambarus clarkii to an acoustic stimulus. J. Exp. Biol. 216, 709-718. Celi, M., Filiciotto, F., Vazzana, M., Arizza,V., Maccarrone, V., Ceraulo, M., Mazzola, S. and Buscaino, G. (2015). Shipping noise affecting immune responses of European spiny lobster Palinurus elephas (Fabricius, 1787). Can. J. Zool. 93, 113-121. Ellers, O. (1995). Discrimination among wave-generated sounds by a swash-riding clam. Biol. Bull. 189: 128–137. Fay, R. R., Popper, A. (1998). Comparative hearing: fish and amphibians, pp. 456. New York: Springer. Filiciotto, F., Vazzana, M., Celi, M., Maccarrone, V., Ceraulo, M., Buffa, G., Di Stefano, V., Mazzola, S. and Buscaino, G. (2014). Behavioural and biochemical stress responses of Palinurus elephas after exposure to boat noise pollution in tank. Mar. Pollut. Bull. 84, 104–114. Mooney, T. A., Hanlon, R., Christensen-Dalsgaard, J., Madsen, P. T., Ketten, D. R. and Nachtigall, P. E. (2012). The potential for sound sensitivity in cephalopods. In The Effects of Noise on Aquatic Life. (ed. A. Popper and A. Hawkins), pp. 125-128. New York: Springer Science+Business Media, LLC. 15 Robson, A., Wilson, R., de Leaniz, C. G. (2007). Mussels Xexing their muscles: a new method for quantifying bivalve behaviour. Mar. Biol. 151: 1195–1204. Simpson, S., Radford, A., Tickle, E., Meekan, M. and Jeffs, A. (2011). Adaptive Avoidance of Reef Noise. PLoS ONE 6, e16625. Vermeij, M. J. A., Marhaver, K. L., Huijbers, C. M., Nagelkerken, I. and Simpson, S. D. (2010). Coral larvae move toward reef sounds. PLoS ONE 5, e10660. Webster, D. B., Fay, R. R. and Popper, A. N. (1992). The evolutionary biology of hearing, pp. 591. New York: Springer-Verlag.
Subjects: 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia > 577 Ecologia
Depositing User: Dr. Francesco Filiciotto
Date Deposited: 09 Sep 2015 08:22
Last Modified: 09 Sep 2015 08:22
URI: http://eprints.bice.rm.cnr.it/id/eprint/11593

Actions (login required)

View Item View Item