Microbiological quality control of single-walled carbon-nanotubes–coated surfaces experimentally contaminated

Natalizi, T. and Frioni, A. and Passeri, D. and Pantanella, F. (2013) Microbiological quality control of single-walled carbon-nanotubes–coated surfaces experimentally contaminated. Il nuovo cimento C, 36 (2). pp. 97-102. ISSN 1826-9885

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The emergence of new nanotechnologies involves the spreading of nanoparticles in various fields of human life. Nanoparticles in general and, more specifically, carbon nanotubes have been adopted for many practical approaches i.e.: coatings for medical devices, food process industry and drug delivery. Humans will be increasingly exposed to nanoparticles but the susceptibility of nanostructured materials to microbial colonization in process of manufacturing and storage has not been thoroughly considered. Therefore, the microbiological quality control of nanoparticles plays a pivotal role. Different analytical methods have been attempted for detecting bacterial population contaminating a surface, but no one can be considered fully appropriate. Here, BioTimer Assay (BTA) and conventional sonication followed by colony forming units method (S-CFU) were applied for microbiological quality control of single-walled carbon nanotubes (SWCNTs)-coated surfaces experimentally contaminated with Streptococcus mutans and Pseudomonas aeruginosa. Our results demonstrated that S-CFU is unreliable to actually determine the number of bacteria, contaminating abiotic surfaces, as it does not detach all adherent bacteria and kills part of the bacterial population. Instead, BTA is a reliable method to enumerate bacteria colonizing SWCNTs-coated surfaces and can be considered a useful tool for microbiological quality control of nanomaterials for human use.

Item Type: Article
Uncontrolled Keywords: Multicellular phenomena, biofilms ; Atomic force microscopy (AFM) ; Structure of carbon nanotubes, boron nanotubes, and other related systems
Subjects: 500 Scienze naturali e Matematica > 530 Fisica
Depositing User: Marina Spanti
Date Deposited: 05 May 2020 12:33
Last Modified: 05 May 2020 12:33
URI: http://eprints.bice.rm.cnr.it/id/eprint/18251

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