The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum

Tang, G. and Zhang, C. and Ju, Z. and Zheng, S. and Wen, Z. and Xu, S. and Chen, Y. and Ma, Z. (2018) The mitochondrial membrane protein FgLetm1 regulates mitochondrial integrity, production of endogenous reactive oxygen species and mycotoxin biosynthesis in Fusarium graminearum. Molecolar Plant Patology, 9 (7). pp. 1595-1611.

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Abstract

Deoxynivalenol (DON) is a mycotoxin produced in cereal crops infected with Fusarium graminearum. DON poses a serious threat to human and animal health, and is a critical virulence factor. Various environmental factors, including reactive oxygen species (ROS), have been shown to interfere with DON biosynthesis in this pathogen. The regulatory mechanisms of how ROS trigger DON production have been investigated extensively in F. graminearum. However, the role of the endogenous ROS-generating system in DON biosynthesis is largely unknown. In this study, we genetically analysed the function of leucine zipper-EF-hand-containing transmembrane 1 (LETM1) superfamily proteins and evaluated the role of the mitochondrial-produced ROS in DON biosynthesis. Our results show that there are two Letm1 orthologues, FgLetm1 and FgLetm2, in F. graminearum. FgLetm1 is localized to the mitochondria and is essential for mitochondrial integrity, whereas FgLetm2 plays a minor role in the maintenance of mitochondrial integrity. The ΔFgLetm1 mutant demonstrated a vegetative growth defect, abnormal conidia and increased sensitivity to various stress agents. More importantly, the ΔFgLetm1 mutant showed significantly reduced levels of endogenous ROS, decreased DON biosynthesis and attenuated virulence in planta. To our knowledge, this is the first report showing that mitochondrial integrity and endogenous ROS production by mitochondria are important for DON production and virulence in Fusarium species.

Item Type: Article
Uncontrolled Keywords: FgLetm1; Fusarium graminearum; endogenous reactive oxygen species; mitochondrial integrity; mycotoxin; virulence
Subjects: 600 Tecnologia - Scienze applicate > 630 Agricoltura e tecnologie connesse > 634 Piante da frutto (Frutticoltura), frutta, scienze forestali
Depositing User: Mr Pasquale Del Vecchio
Date Deposited: 06 May 2019 08:49
Last Modified: 06 May 2019 09:12
URI: http://eprints.bice.rm.cnr.it/id/eprint/18876

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