Mazzeo, Maria Fiorella and Sorrentino, Alida and Gaita, Marcello and Cacace, Giuseppina and Di Stasio, Michele and Facchiano, Angelo and Comi, Giuseppe and Malorni, Antonio and Siciliano, Rosa Anna (2006) Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms. Applied and environmental microbiology, 72 (2). pp. 1180-1189. ISSN 0099-2240
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2006 - Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms.pdf - Published Version Download (158kB) | Preview |
Abstract
A methodology based on matrix-assisted laser desorption ionization–time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria.
| Item Type: | Article |
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| Subjects: | 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia |
| Depositing User: | Ing. Michele Di Stasio |
| Date Deposited: | 12 Jan 2017 11:10 |
| Last Modified: | 12 Jan 2017 11:10 |
| URI: | http://eprints.bice.rm.cnr.it/id/eprint/15350 |
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