Computerized sperm motility analysis in toxicity bioassays: A new approach to pore water quality assessment

Fabbrocini, Adele and Di Stasio, Michele and D’Adamo, Raffaele (2010) Computerized sperm motility analysis in toxicity bioassays: A new approach to pore water quality assessment. Ecotoxicology and Environmental Safety, 73 (7). pp. 1588-1595. ISSN 0147-6513

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Abstract

The aim of this study was to test the sensitivity of computerized sperm motility analysis in the sea urchin Paracentrotus lividus as the endpoint in toxicity bioassays. The tested matrices were pore water samples collected in an agriculture-impacted Mediterranean lagoon, Lake Varano (Italy). Two standardized bioassays were also conducted as controls, the P. lividus spermiotoxicity test and the Vibrio fischeri (Microtoxs) test. VCL (curvilinear velocity), VSL (straight line velocity), VAP (average path velocity), and the percentage of rapid spermatozoa recorded by the Sperm Class Analyzers system showed high sensitivity and discrimination ability, to a degree comparable with the larval development endpoint of the spermiotoxicity test. The test evaluated in this study requires small volumes of matrices, involves minimal sample manipulation, and can easily be extended to many other bioindicator species. It may therefore be considered a promising ‘‘quick response tool’’ following hazardous events that may adversely affect an aquatic ecosystem.

Item Type:	Article
Uncontrolled Keywords:	Toxicity bioassays, Sea urchin, Computerized sperm motility analysis, Embryonic development, Microtoxs, Pore water, Mediterranean lagoon
Subjects:	500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia
Depositing User:	Ing. Michele Di Stasio
Date Deposited:	12 Jan 2017 12:49
Last Modified:	12 Jan 2017 12:49
URI:	http://eprints.bice.rm.cnr.it/id/eprint/15369

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