Cittaro, Davide and Lampis, Valentina and Luchetti, Alessandra and Coccurello, Roberto and Guffanti, Alessandro and Felsani, Armando and Moles, Anna and Stupka, Elia and D’ Amato, Francesca R. and Battaglia, Marco (2016) Histone Modifications in a Mouse Model of Early Adversities and Panic Disorder: Role for Asic1 and Neurodevelopmental Genes. Scientific Reports, 6. p. 25131. ISSN 2045-2322
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Histone Modifications in a Mouse Model of Early Adversities and Panic Disorder Role for Asic1 and Neurodevelopmental Genes.pdf - Published Version Download (1MB) | Preview |
Abstract
Hyperventilation following transient, CO2-induced acidosis is ubiquitous in mammals and heritable. In humans, respiratory and emotional hypersensitivity to CO2 marks separation anxiety and panic disorders, and is enhanced by early-life adversities. Mice exposed to the repeated cross-fostering paradigm (RCF) of interference with maternal environment show heightened separation anxiety and hyperventilation to 6% CO2-enriched air. Gene-environment interactions affect CO2 hypersensitivity in both humans and mice. We therefore hypothesised that epigenetic modifications and increased expression of genes involved in pH-detection could explain these relationships. Medullae oblongata of RCF- and normally-reared female outbred mice were assessed by ChIP-seq for H3Ac, H3K4me3, H3K27me3 histone modifications, and by SAGE for differential gene expression. Integration of multiple experiments by network analysis revealed an active component of 148 genes pointing to the mTOR signalling pathway and nociception. Among these genes, Asic1 showed heightened mRNA expression, coherent with RCF-mice’s respiratory hypersensitivity to CO2 and altered nociception. Functional enrichment and mRNA transcript analyses yielded a consistent picture of enhancement for several genes affecting chemoception, neurodevelopment, and emotionality. Particularly, results with Asic1 support recent human findings with panic and CO2 responses, and provide new perspectives on how early adversities and genes interplay to affect key components of panic and related disorders.
| Item Type: | Article |
|---|---|
| Subjects: | 500 Scienze naturali e Matematica 500 Scienze naturali e Matematica > 570 Scienze della vita; Biologia |
| Depositing User: | PhD Roberto Coccurello |
| Date Deposited: | 19 Dec 2016 14:04 |
| Last Modified: | 19 Dec 2016 14:04 |
| URI: | http://eprints.bice.rm.cnr.it/id/eprint/15458 |
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