Tendon cell ciliary length as a biomarker of in situ cytoskeletal tensional homeostasis

Lavagnino, Michael and Gardner, Keri and Sedlak, Aleksa Michele and Arnoczky, Steven Paul (2013) Tendon cell ciliary length as a biomarker of in situ cytoskeletal tensional homeostasis. M.L.T.J. Muscles, Ligaments and Tendons Journal, 3 (3). pp. 118-121. ISSN 2240-4554

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Abstract

To determine if tendon cell ciliary length could be used as a biomarker of cytoskeletal tensional homeostasis, 20 mm long adult rat tail tendons were placed in double artery clamps set 18 mm apart to create a 10% laxity. The tendons were allowed to contract over a 7 day period under culture conditions. At 0, 1, 5, and 7 days the tendon cell cilia were stained and ciliary length measured using confocal imaging. There was a significant (p<0.001) increase in ciliary length at 1 day. At day 5 (when the tendon became visibly taut) there was a significant (p<0.001) decrease in ciliary length compared to day 1. By day 7 the tendon remained taut and ciliary length returned to day zero values (p=0.883). These results suggest that cilia length reflects the local mechanobiological environment of tendon cells and could be used as a potential in situ biomarker of altered cytoskeletal tensional homeostasis

Item Type:	Article
Uncontrolled Keywords:	Tendon, cilia, recalibration, tensional homeostasis
Subjects:	600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.7 Malattie del sistema muscoloscheletrico
Depositing User:	Danilo Dezzi
Date Deposited:	17 Apr 2014 14:17
Last Modified:	17 Apr 2014 14:17
URI:	http://eprints.bice.rm.cnr.it/id/eprint/8250

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