The use of mouse ribs in organ culture improves the in vitro bone resorption assay

Zonefrati, Roberto and Tanini, Annalisa and Bartaloni, Jacopo and Tognarini, Isabella and Galli, Gianna and Carossino, Annamaria and Brandi, Maria Luisa (2005) The use of mouse ribs in organ culture improves the in vitro bone resorption assay. Clinical cases in mineral and bone metabolism, 2 (1). pp. 47-51. ISSN 1971-3266

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Abstract

This study investigated in vitro bone resorption determining the calcium release in ribs, long bone and calvaria prelabelled with 45Ca from 17 day mouse fetuses, both in the absence and in the presence of specific stimuli, such as parathyroid hormone and calcitonin. 10- 7 M rat parathyroid hormone (1-34) (rPTH (1-34)) stimulated bone resorption (evaluated through the ratio treated ribs/control ribs) in 93% of the organ cultures, while lower success rate was obtained using calvaria and long bone from the same animals. In the absence of test substances, no differences were observed in paired ribs from the same fetus, while corresponding ribs from different fetuses showed considerable differences. Within every single hemythorax, bone resorption varies according to the rib position either in control ribs, or in those ones treated with rPTH (1-34). In the presence of rPTH (1-34), bone resorption showed to be dose-dependent producing a maximal response at 10- 6 M, a minimal response at 10- 8 M and a half-maximal response at 5x10-8 M. Salmon calcitonin (sCT) had no effect upon basal resorption, while it acted as a potent inhibiting agent in PTH response. The conspicuous number of samples which can be obtained from a relatively low number of mice, the reproducibility of results, together with the hormone sensitivity make the fetal mouse rib model an excellent tool for evaluating bone resorption in vitro

Item Type:	Article
Subjects:	600 Tecnologia - Scienze applicate > 610 Medicina e salute (Classificare qui la tecnologia dei servizi medici) > 616 Malattie (classificare qui la Clinica medica, la medicina basata sull'evidenza, la Medicina interna, la Medicina sperimentale) > 616.7 Malattie del sistema muscoloscheletrico
Depositing User:	Danilo Dezzi
Date Deposited:	24 Apr 2014 10:42
Last Modified:	24 Apr 2014 10:42
URI:	http://eprints.bice.rm.cnr.it/id/eprint/8824

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